l-Asparaginase fromE. coli
نویسندگان
چکیده
منابع مشابه
L-Asparaginase from escherichia coli B. Succinylation and subunit interactions.
Asparaginase from Esckerickio coli B has been modified with increasing amounts of [r4C]succinic anhydride. Enzyme activity is enhanced when 15 % and 25 % of the lysyl residues are succinylated. Up to 40% of the lysyl residues were succinylated without destroying enzyme activity and without causing dissociation of this oligomeric protein. More extensive succinylation causes dissociation into sub...
متن کاملCrystal packing of plant-type L-asparaginase from Escherichia coli.
Plant-type L-asparaginases hydrolyze the side-chain amide bond of L-asparagine or its beta-peptides. They belong to the N-terminal nucleophile (Ntn) hydrolases and are synthesized as inactive precursor molecules. Activation occurs via the autoproteolytic release of two subunits, alpha and beta, the latter of which carries the nucleophile at its N-terminus. Crystallographic studies of plant-type...
متن کاملProduction of L-asparaginase II by Escherichia coli.
l-Asparaginase II was synthesized at constant rates by Escherichia coli under anaerobic conditions. The enzyme was produced optimally by bacteria grown between pH 7 and 8 at 37 C. Although some enzyme was formed aerobically, between 100 and 1,000 times more asparaginase II was produced during anaerobic growth in media enriched with high concentrations of a variety of amino acids. Bacteria grown...
متن کاملProduction and optimization of L-asparaginase in Escherichia coli
-ASPARAGINASE (L-ASNase) has been widely used as a therapeutic agent in the treatment for various lymphoblastic leukemia diseases. This study aimed to isolate and purify local bacterial isolates that are capable of producing L-ASNase, so 150 bacterial isolates from the Nile River where isolated, purified and their ability to produce L-ASNase was assessed. Among these isolates, 32 bacterial isol...
متن کاملL-asparagine depletion and L-asparaginase activity in children with acute lymphoblastic leukemia receiving i.m. or i.v. Erwinia C. or E. coli L-asparaginase as first exposure.
PURPOSE The present study was aimed at investigating L-asparaginase (L-ASE) activity (in plasma) and L-asparagine (L-ASN) depletion (in plasma and CSF) in children with newly diagnosed acute lymphoblastic leukemia (ALL) exposed for the first time to different L-ASE products. PATIENTS AND METHODS During the induction treatment of the AIEOP ALL 95 study, 62 patients were treated with either Erw...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1971
ISSN: 0002-1369
DOI: 10.1080/00021369.1971.10859899